T Cell Activation Cell and Cytokine Profiling Kit

Key Benefits

• Speed: Sample to actionable data by allowing multiple assays to be run in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
• Results: 15 endpoints from a single well assay measuring T cell phenotypes, early and late cell activation markers in each phenotype, cell viability, cell count, proliferation and secreted effector cytokines.
• Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
• Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.

Kit Description

The T Cell Activation Cell and Cytokine Profiling Kit assesses the activation status of T cells while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with a fluorescent Cell Membrane Integrity dye.
• Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
• The T cell phenotypes are profiled for the expression of 3 activation markers, CD69 (early), CD25 (late), and HLA-DR (even later, with strong stimulation).
• Optimized workflow enables the measurement of effector cytokines secreted by activated T cells,Th1 cytokine IFNγ/ multifunctional cytokine TNFα in the same assay well.

Principle

The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.

Workflow