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Mitochondrial Depolarization Kit consists of a dye that detects mitochondrial depolarization by leaking from the lumen of mitochondria to the cytoplasm. This causes the cell to exhibit decreased fluorescence. This kit can be multiplexed with other IntelliCyt kits.
Mitochondrial membrane potential is determined by sequestration of a small fluorescent molecule inside the lumen of intact mitochondria with an active membrane potential. Upon mitochondrial depolarization the dye leaks into the cytoplasm and the cell exhibits a decrease in fluorescence.
Figure 1: Identify Cells with Depolarized Mitochondrial Membranes. Mitochondrial depolarization will cause leakage of the dye out of the mitochondria, and the fluorescence intensity of the cell will decrease. Adjust the gate as necessary to encompass the entirely of the left (depolarized) peaks. Depending on the degree of mitochondrial damage, multiple peaks within the “mito damage” gate are possible.
|Dimensions||15 × 13 × 8 in|