Human NK Cell Mediated Killing Kit

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Key Benefits


  • Speed: Sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
  • Results: Nine endpoints from a single well assay measuring activation markers (CD69 and CD25), cell membrane integrity, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic protease Granzyme B.
  • Biological insights: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
  • Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.

Kit Description


The Human General Immune Cell Mediated Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye.
  • Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
  • NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells.
  • The panel also includes 2 different NK cell functional activation markers, CD69 and CD25.
  • Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

Workflow


Workflow

Additional information

Weight 8 lbs
Dimensions 8 × 15 × 13 in

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