Human General Immune Cell Mediated Killing Kit

Key Benefits

• Speed: Expedient sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
• Results: Five endpoints from a single well assay measure target and effector cells, mitochondrial membrane depolarization, cell membrane integrity, cell count, and secreted pro-apoptotic protease Granzyme B.
• Biological insights: Investigation of multiple mechanisms of immune cell mediated cell killing in one well.
• Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.

Kit Description

The Human General Immune Cell Mediated Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

• Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye and mitochondrial membrane potential dye.
• Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
• Simultaneous analysis of multiple mechanisms of immune cell mediated cell killing in a single, high content assay including mitochondrial membrane depolarization, loss of cell membrane integrity, cell count, and pro-apoptotic protease Granzyme B.

Workflow