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The Cell Proliferation and Encoding Dye V/Blue (Tag-it VioletTM) provides a straightforward workflow to simultaneously measure cell proliferation or multiple encoded cell populations. Enable labeling of many standard cell lines by using different intensities of a single fluorophore. Proliferated cells are distinguished from non-proliferated cells by decreased fluorescence intensity.
Proliferation Use: The principle of dye dilution. Cell proliferation is detected and quantified based on the halving or “dilution” of the loaded dye after each round of cellular division. Through subsequent cell divisions, daughter cells retain approximately half the fluorescent label of the parent cells.
Encoding Use: Multiple cell populations are stained with different intensities of dye, distinguish up to 4 cell populations (Bright, Mid, Dim and Unstained). Target cells are stained with different concentrations of V/Blue encoding dye in order to discriminate the different cell populations.
Figure 1: Data for encoding. The Bright, Mid, Dim, and Unstained populations can be separated with the brightest population furthest to the right.
Figure 2. Data for proliferating cells. Draw a gate on the unstained cells (furthest to the left) and the non-proliferated cells (furthest to the right). Draw a gate on the proliferated cells between the two gates.
|Dimensions||15 × 13 × 8 in|
5 x 384, 20 x 384, 50 x 384