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Each Cell Membrane Integrity Kit consists of a spectrally distinct proprietary dye that is taken up by cells with compromised membranes and excluded by healthy cells with intact membranes The Cell Membrane Integrity Kits can be multiplexed with other IntelliCyt reagents.
The Cell Membrane Integrity dyes are fluorescent molecules that are cell impermeant. Healthy cells with intact cell membranes will exclude the dyes. Unhealthy cells with compromised membranes will allow entry of the dye into the intracellular space. Upon entry to the cell, the dye localizes to the nucleus and binds to DNA by intercalation.
Figure 1: Identify Non-Viable Cells. Binding of a DNA dye to the cells gives a measurement of cell viability in the histogram. Positive cells (right peak) represent the non-viable population. Adjust the gate as necessary to encompass this peak. This gate will be used to report the percentage of non-viable cells in each well.
|Dimensions||15 × 13 × 8 in|