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The Cell Cycle Kit utilizes a DNA binding dye to enable the creation of a cell cycle profile and the quantification of the relative percentage of cells in each phase of the cell cycle.
Cells stained with the cell cycle dye will fluoresce in proportion to their DNA content. The cell cycle consists of four distinct phases: G0/G1 phase (growth), S phase (synthesis), G2 phase and M phase (mitosis). Each phase can be identified based on the relative amounts of DNA resident in the cell, with G0/G1 cells having the least amount of DNA and G2/M cells with the most. These relative differences in DNA content are easily detected by fluorescence intensity, and can be utilized to establish quantitative cell cycle profiles.
The Cell Cycle Kit utilizes a proprietary cell cycle dye that is able to enter live cells and specifically bind to DNA without need for fixation or permeabilization. The single step addition, no wash assay format has been designed for multiplexing, and is compatible with GFP expression cell lines.
|Dimensions||15 × 13 × 8 in|