Caspase 3/7 (B/Green) Kit

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Key Benefits


  • No wash protocol – just add and read for fast, easily automated workflow.
  • Multiplex with Qbeads PlexScreen kits for richer content.

Kit Description


Caspase 3/7 Detection Kit consists of a dye that fluoresces when cleaved by activated caspases 3 & 7 which play an important role in the apoptotic pathway. An increase in fluorescence scores as a positive for apoptotic response. This kit can be multiplexed with other IntelliCyt kits.

Principle


The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.

Chart Image

Figure 1: Identify Cells Positive for Caspase. Caspase activation is shown in the histogram. Adjust the gate as necessary to encompass the entirety of the right (positive) peak. This gate will be used to report the percentage of Caspase positive cells in each well.

Additional information

Weight 8 lbs
Dimensions 15 × 13 × 8 in

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