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Caspase 3/7 Detection Kit consists of a dye that fluoresces when cleaved by activated caspases 3 & 7 which play an important role in the apoptotic pathway. An increase in fluorescence scores as a positive for apoptotic response. This kit can be multiplexed with other IntelliCyt kits.
The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.
Figure 1: Identify Cells Positive for Caspase. Caspase activation is shown in the histogram. Adjust the gate as necessary to encompass the entirety of the right (positive) peak. This gate will be used to report the percentage of Caspase positive cells in each well.
|Dimensions||15 × 13 × 8 in|