Antibody Internalization Mouse Reagent

Key Benefits

• No wash protocol- fast, easy antibody-labeling method optimal for screening antibody internalization
• High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 20 µL of cells and antibody.
• Cell viability can also be measured by multiplexing Intellicyt® Cell Membrane Integrity Dye to assess general cell health and antibody function.
• Cell specificity can be characterized by multiplexing Intellicyt® Encoding dyes (cell lines) or directly conjugated fluorescent antibodies (cells).
• The Qbeads® Assay can also be multiplexed to perform more detailed antibody assessments on the sample, such as cytokine release.
• Available in Human and Mouse formats

Kit Description

The Intellicyt Antibody Internalization is designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 20 µL of cells and antibody.

Principle

Antibodies are easily labeled with Intellicyt Antibody Internalization Reagent, a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.