Do you have an Intellicyt eCommerce account?
Sign in to see the price of this product and make an online purchase.
Currently, the Intellicyt eCommerce solution is available for US customers only.
The 4-Plex Apoptosis Kit is a multi-parameter assay consisting 4 different apoptosis measurements per well (cell viability, caspase 3/7 activity, Annexin V binding, and mitochondrial depolarization) for a robust determination of apoptosis progression and insight into mechanism of action. The individual reagent components are also available separately for greater flexibility.
The activation of Caspase 3 and 7 is detected by the use of NucView 488 Caspase-3/7 substrate, which upon cleavage by activated enzyme, results in an intracellular fluorescent signal.
Surface expression of phosphatidylserine is detected by the binding of Annexin-V to the cell surface.
Cell viability is determined by the ability of live cells to exclude a DNA binding.
Mitochondrial membrane potential is determined by sequestration of a small fluorescent molecule inside the lumen of intact mitochondria with an active membrane potential. Upon mitochondrial depolarization the dye leaks into the cytoplasm and the cell exhibits a decrease in fluorescence.
*Apoptosis reagents can be ordered separately for custom multiplexing.
|Dimensions||15 × 13 × 8 in|